Apoptosis is a naturally occurring process by which a cell is directed to Programmed Cell Death. Apoptosis is based on a genetic program that is an indispensable part of the development and function of an organism. Inappropriately regulated apoptosis is implicated in disease states. Apoptosis has become one of the most active research areas in the life sciences.
In normal viable cells, phosphatidylserine(PS) is located on the cytoplasmic surface of the cell membrane. However, in apoptotic cells, PS is translocated from the inner to the outer of the cell membrane, thus exposing PS to the external cellular environment. The annexin V is a phospholipid-binding protein that has a high affinity for PS. Annexin V labeled with a fluorophore or biotin can identify apoptotic cells by binding to PS exposed on the outer surface of the cell membrance. Highly fluorescent annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, one of the earliest indicators of apoptosis.
Propidium Iodide（PI）is a nucleic acid dyes that can stain dead cell but can not pass through the live cells, thereby separating apoptosis from necrosis.
• Fast :Procedure takes approximately in 10 minutes.
• Compared with DNA detection methods, apoptotic cells can be detected earlier.
• Accurate: Accurately distinguish normal cells, early apoptotic cells, late apoptotic cells and necrotic cells, and count the proportion of cells in each group.